A Detailed Analysis of the Negative Effects of Feminizing HRT
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Negative Effects of Feminizing Hormone Therapy (Transfeminine HRT)
Hormone therapy for transfeminine individuals (male at birth, female gender identity) can profoundly alter physiology. Along with its benefits, it carries certain risks. Below we analyze key negative effects, comparing transfeminine individuals on HRT to cisgender women (and cisgender men where relevant), and discuss mechanisms and evidence. We also review strategies to mitigate these risks.
Breast Cancer Risk
Incidence and Comparison: Transfeminine individuals on long-term estrogen therapy do face a risk of breast cancer, but data suggest it remains markedly lower than in cisgender women. A large Dutch cohort found transfeminine patients had a 46-fold higher breast cancer incidence than cis men, yet only about 30% of the risk observed in cis women (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed). In that study (median 18 years of HRT), 15 breast cancer cases occurred in ~2,260 trans women, yielding a standardized incidence ratio (SIR) of 0.3 relative to cis women (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed). This means trans women’s breast cancer risk, while elevated above cis men’s (who rarely develop breast cancer), is substantially lower than a cis female’s lifetime risk.
Mechanisms: The development of breast tissue under estrogen stimulation is the primary driver. Exogenous estrogen (often with an anti-androgen or progestin) promotes ductal growth and breast cell proliferation, which over years can lead to malignant changes (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed). Notably, tumors seen in trans women are usually estrogen receptor (ER) and progesterone receptor positive (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed), similar to typical postmenopausal breast cancers. The reduced overall risk compared to cis women may reflect later age of breast development (adult onset of breast tissue under HRT) and possibly lower cumulative estrogen exposure over the lifespan.
Evidence and Guidelines: Because risk is not negligible, many experts recommend breast cancer screening for trans women on HRT akin to cis women, especially after many years on estrogen or over age ~50. Current guidelines suggest following cisgender female screening protocols (e.g. mammography starting at age 50 or earlier if risk factors), given documented cases (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed). Ongoing research is examining if specific regimens (e.g. inclusion of progestins) influence tumor risk. Overall, while feminizing HRT raises breast cancer risk above male baseline, it still appears lower than in cis females, and routine monitoring can help early detection.
Venous Thromboembolism (VTE: DVT and Pulmonary Embolism)
Risk Level: Estrogen has well-known pro-thrombotic effects. Transfeminine hormone therapy is associated with an increased risk of venous thromboembolism (blood clots in deep veins and lungs). Multiple studies show higher VTE rates in trans women on estrogen compared to both cis men and cis women. For example, a large cohort analysis found trans women on oral estrogen had about a 2.7-fold higher hazard of VTE compared to cisgender women ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). Similarly, relative to cis men, the risk may be several-fold higher; one analysis noted VTE occurred in ~5% of trans women after ~7.7 years of estrogen, equating to ~355% higher risk than in cis men (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)). In absolute terms, the incidence is modest (e.g. ~2 events per 1000 person-years in one meta-analysis ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC )), but clearly elevated on a relative basis.
Contributing Factors: The route and type of estrogen play a significant role. Older regimens using ethinyl estradiol (a synthetic oral estrogen once used for transgender care) had especially high thrombotic risk. After clinics shifted to bioidentical 17β-estradiol, VTE rates dropped significantly (Overview of feminizing hormone therapy | Gender Affirming Health Program). Even with estradiol, oral administration undergoes first-pass liver metabolism, increasing production of clotting factors and lowering anticoagulant proteins ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). This pro-coagulant shift (elevated factors II, VII, IX, XI and decreased protein C, S, antithrombin) promotes thrombosis ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). By contrast, transdermal estrogen (patches/gels) avoids high liver first-pass concentrations and appears to minimize VTE risk – data in menopausal women show no significant clot risk with transdermal estradiol (Overview of feminizing hormone therapy | Gender Affirming Health Program), and transgender studies similarly report no VTE increase on transdermal formulations ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ).
Other factors include dosage (higher estrogen levels correlate with more risk), additional medications (the anti-androgen cyproterone can independently raise clot risk (Cyproterone acetate and the risk of meningioma)), and baseline patient risk factors (age, obesity, smoking, genetic thrombophilia). Notably, smoking synergistically increases estrogen’s thrombotic risk. All trans women who smoke should be counseled on tobacco cessation at every visit, as smoking while on estrogen further elevates VTE and cardiovascular risk (Overview of feminizing hormone therapy | Gender Affirming Health Program). Still, guidelines note that smoking status shouldn’t categorically bar someone from HRT, but rather prompt risk mitigation (e.g. using transdermal estrogen and encouraging quitting) (Overview of feminizing hormone therapy | Gender Affirming Health Program).
Clinical Manifestations: The VTE risk on feminizing hormones most commonly presents as deep vein thrombosis (DVT) in the legs or pulmonary embolism (PE). Symptoms like unilateral leg swelling/pain or sudden shortness of breath in a trans woman on HRT should raise suspicion. Fortunately, while the relative risk is higher, the absolute risk remains low for most individuals ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). For perspective, one meta-analysis of ~11,500 transfeminine patients on oral estrogen found an incidence around 2 events per 1000 person‐years ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). This is comparable to or lower than clot risk seen in cisgender women on oral contraceptives or postmenopausal HRT. Nonetheless, providers remain vigilant: estrogen-related VTE is one of the most common serious complications in transfeminine care ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ).
Mechanism: Estrogen induces a hypercoagulable state. Within a year of starting HRT, studies document increases in clotting Factors (like IX, XII) and reductions in anticoagulants (protein C, antithrombin) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). These changes mirror those in cis women on the Pill or pregnancy. Additionally, estrogen can raise levels of fibrinogen and plasminogen activator inhibitor, tilting the balance toward clot formation. This mechanism explains why risk is higher with oral estrogen (greater hepatic impact). The form of estrogen matters too: ethinyl estradiol is potent in liver effect (and is now generally avoided in transfeminine therapy due to this), whereas transdermal 17β-estradiol has minimal impact on coagulation proteins (Overview of feminizing hormone therapy | Gender Affirming Health Program).
In summary, transfeminine HRT roughly doubles to triples VTE risk relative to cis women (and even more so relative to cis men) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)). The risk is driven by estrogen’s pro-thrombotic effects, especially with oral dosing. Proper route selection and patient counseling can greatly mitigate this risk (detailed under Risk Mitigation).
Cardiovascular Disease: Stroke and Myocardial Infarction
Ischemic Stroke: Estrogen-related clotting and possibly blood pressure changes can also increase the risk of ischemic stroke (cerebral infarction). Current evidence suggests transfeminine individuals on hormones have a modestly higher stroke incidence than cisgender peers. A recent meta-analysis reported about a 1.3-fold higher incidence of stroke in trans women vs. cis men (95% CI up to 1.8) (Importance of Sex and Gender Differences in Enrollment and ...). Similarly, compared to cis women, trans women may have ~1.8 times the risk of stroke ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). For example, one cohort observed an ischemic stroke rate of ~4.8 per 1000 in trans women, corresponding to an ~80% higher risk than cis men (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)). Another U.S. study (Kaiser Permanente cohort) initially found no overall stroke difference versus controls over ~4 years on average, but in those who had initiated HRT, stroke rates became more pronounced over longer follow-up (Cross-sex Hormones and Acute Cardiovascular Events in Transgender Persons: A Cohort Study - PubMed) (Cross-sex Hormones and Acute Cardiovascular Events in Transgender Persons: A Cohort Study - PubMed). This suggests that cumulative estrogen exposure over many years might be needed to significantly impact stroke risk.
Myocardial Infarction (Heart Attack): The effect of feminizing hormones on heart attack risk is complex. Some data show trans women have more MIs than cis women, but not more than cis men. In one large study, trans women had a hazard ratio of ~1.8 for MI compared to cisgender women (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)). However, their MI risk was similar to cisgender men (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)) (who inherently have higher heart attack rates than cis women). In other words, a trans woman’s cardiovascular risk profile tends to resemble the male baseline from which she started. After transition, her risk may remain closer to her birth sex’s risk or in between male and female norms. A 2019 study in Circulation likewise found trans women had about 2.5 times higher MI risk than cis women, but no significant increase versus cis men ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). This suggests estrogen therapy does not completely negate the prior lifetime effects of testosterone, cholesterol, etc., that influence male-pattern cardiac risk.
Mechanisms: Several mechanisms could contribute to increased stroke and MI risk in transfeminine people:
- Hypercoagulability: As discussed, estrogen’s clotting effects can cause thromboembolic strokes (ischemic strokes due to blood clots in brain arteries) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). Most data point to ischemic stroke risk; there’s little evidence that hemorrhagic (bleeding) stroke is significantly increased, except possibly via hypertension.
- Blood Pressure: Estrogen can affect the renin-angiotensin system and cause fluid retention, potentially raising blood pressure in some individuals. However, findings are mixed; some trans women actually see blood pressure decrease on estrogen (especially if testosterone was contributing to higher BP). One study noted lower systolic BP and improved arterial stiffness in trans women on estrogen, unless a progestin was added ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). So the net effect on BP varies.
- Dyslipidemia: Estrogen typically improves the cholesterol profile (raising HDL, lowering LDL) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). This would be protective for heart disease. Indeed, transgender cohorts often show favorable lipid changes on HRT (akin to cis women’s cardioprotective lipid profile) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). However, weight gain and reduced insulin sensitivity can counteract some benefits ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). Notably, oral estrogen may increase triglycerides significantly, which can elevate pancreatitis risk and may contribute to cardiovascular risk if extreme ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ).
- Body Fat Distribution: With estrogen and androgen blockade, transfeminine individuals tend to gain subcutaneous fat and lose visceral fat, moving toward a “female” fat distribution. This could reduce cardiac risk (since visceral fat is a risk factor). However, overall weight could increase, and lean muscle mass decreases, possibly reducing overall metabolic rate and insulin sensitivity ( Effects of gender-affirming hormone therapy on insulin resistance and body composition in transgender individuals: A systematic review - PMC ).
Because of these mixed influences, the net cardiovascular risk in trans women is an evolving picture. Early research (with short follow-ups) showed minimal differences, but newer long-term data indicate heightened risk of cardiovascular events (stroke, heart attack) after 5–10+ years of HRT (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)) (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)). It’s important to emphasize that even if relative risk is elevated, many trans women will not experience these events, especially if preventive care is in place. The presence of traditional cardiac risk factors (smoking, diabetes, hypertension, family history) likely plays a bigger role in an individual’s MI/stroke risk than HRT itself. Still, clinicians are advised to monitor blood pressure, lipids, and glucose during therapy, and manage them aggressively to offset any hormone-related increase in cardiovascular risk.
Metabolic Effects and Diabetes Risk
Feminizing hormone therapy induces significant metabolic changes. Key among these are effects on body composition and insulin-glucose metabolism, which can influence diabetes risk.
Body Composition: Estrogen plus androgen suppression causes a shift toward a typically female body composition. Transfeminine patients experience decreased lean muscle mass and increased fat mass over time ( Effects of gender-affirming hormone therapy on insulin resistance and body composition in transgender individuals: A systematic review - PMC ). Prospective studies show loss of muscle bulk (on average a few kilograms of lean mass in the first year) and a relative increase in body fat percentage. In fact, trans women’s body fat percentage tends to rise into the range of cis women, while muscle mass drops partway toward female norms ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). For example, one study found adolescent trans women on estradiol had ~31% body fat vs ~28% pre-HRT (and cis men ~20%, cis women ~35%) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). This redistribution (more subcutaneous fat on hips/thighs, less visceral abdominal fat) can have mixed metabolic implications: more subcutaneous fat is metabolically benign, but loss of muscle can reduce basal metabolism and glucose uptake.
Insulin Sensitivity: Evidence suggests feminizing HRT can worsen insulin sensitivity in many patients. A systematic review reported that 5 of 8 studies on trans women found increased insulin resistance after starting estrogen (the others found no change) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). In one controlled experiment, insulin sensitivity (measured by glucose tolerance tests) decreased in trans women on estrogen ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). Trans women have been found to be significantly more insulin resistant than cis men; one study noted a lower insulin sensitivity index in trans women vs male controls (0.078 vs 0.142 mL/μU, P = 0.011) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). The likely causes are the reduction in muscle mass (a major site of glucose disposal) and gain in fat mass, along with direct hormonal effects. Estrogen can affect how muscle and liver respond to insulin – some data from cisgender research suggest high doses of estrogen may impair insulin’s action on muscle. Additionally, use of certain anti-androgens (like high-dose cyproterone) might contribute to weight gain and insulin resistance, though data are limited.
Glucose and Diabetes Risk: With increased insulin resistance, the risk of developing type 2 diabetes could rise for trans women on long-term HRT. However, hard outcomes data are still emerging. Some population studies hint at a trend toward higher diabetes prevalence in transfeminine cohorts, but confounders (like weight and lifestyle) are hard to parse. It’s noteworthy that testosterone (in cis men) tends to promote insulin resistance, whereas estrogen in cis women is generally insulin-sensitizing – yet in trans women, the scenario is unique because the intervention (adding estrogen + blocking testosterone) is abrupt and often leads to weight gain. The World Professional Association for Transgender Health (WPATH) notes that metabolic syndrome features should be monitored during feminizing therapy. Clinicians often check fasting glucose or A1c periodically. While no dramatic spike in diabetes incidence has been proven, early studies indicate a potential uptick in risk ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). For instance, one analysis observed higher odds of metabolic syndrome components in trans women on HRT, including elevated fasting glucose and triglycerides (though not always reaching diabetic range).
Lipid Profile: As a brief aside, estrogen’s effect on blood lipids in trans women appears generally favorable:
- Estradiol raises HDL (“good” cholesterol) and lowers LDL, similar to effects in cisgender women ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ).
- Total cholesterol often modestly decreases ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ).
- Triglycerides (TG) can increase, especially on oral estrogen ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ) ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). A meta-analysis found trans women’s TG rose significantly after ≥24 months of oral HRT, whereas those on transdermal estrogen did not have this increase ( Cardiovascular Risk in Transgender People With Gender-Affirming Hormone Treatment - PMC ). Marked hypertriglyceridemia is uncommon but has been reported in some trans women on high-dose oral estradiol, occasionally leading to acute pancreatitis (Acute pancreatitis due to hypertriglyceridaemia in a transgender ...) (a rare complication).
In summary, feminizing HRT may reduce insulin sensitivity and increase body fat, potentially elevating the long-term risk of type 2 diabetes. It’s recommended to monitor weight, blood sugar, and cholesterol during therapy. Encouraging a healthy diet and regular exercise is important to counteract these metabolic shifts. Some trans women actually improve their lifestyle (diet/exercise) during transition, which can offset HRT effects. Thus, with good preventive care, many metabolic changes can be managed such that the net health impact is minimized.
Hyperprolactinemia and Prolactinoma
Prolactin Changes: Estrogens stimulate the pituitary gland’s lactotroph cells. Many trans women on HRT develop elevated prolactin levels (hyperprolactinemia), especially when high estrogen doses are used in combination with certain antiandrogens. Typical physiologic prolactin in adult males is relatively low; under estrogen exposure, prolactin often rises into the female reference range or above. Studies have documented prolactin increases of over 100% from baseline in trans women on estradiol, particularly when combined with cyproterone acetate (a potent antiandrogen with progestogenic activity) ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ) ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ). For example, one study observed median prolactin jumping from ~8 ng/mL to ~19 ng/mL (a 149% increase) over 12 months with estrogen + cyproterone, whereas estrogen + spironolactone caused only a ~35–45% increase ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ) ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ). This indicates cyproterone amplifies prolactin elevation relative to other blockers.
Prolactinoma Risk: The critical question is whether these prolactin rises lead to prolactinomas – benign pituitary tumors. Fortunately, prolactinomas in transfeminine individuals are rare. Current guidelines (WPATH and Endocrine Society) note that only a few case reports of prolactinomas in trans women have been published, and no increase in tumor incidence was seen in large cohorts (). In one series from the Netherlands involving thousands of trans women on long-term HRT, clinically significant prolactinomas were not observed, despite hyperprolactinemia being common in those on cyproterone. As the Endocrine Society states: “Given that only a few case studies reported prolactinomas, and prolactinomas were not reported in large cohorts of estrogen-treated persons, the risk is likely to be very low.” (). In other words, while many trans women get elevated blood prolactin, it usually does not progress to a pituitary adenoma.
Cases and Severity: The few documented cases of prolactinoma in transfeminine patients typically involved high-dose estrogen use over many years (often non-standard doses or older estrogen formulations) or the combination of estrogen + cyproterone. These patients presented with symptoms like headaches, visual field changes (from optic chiasm compression), or galactorrhea (milk discharge), similar to prolactinoma presentations in other populations (Prolactinoma in a Male to Female Transgender Woman on Gender ...). Treatment (dopamine agonists like bromocriptine or surgery in large tumors) has been successful in the reported cases. The prolactinomas were generally benign microadenomas. So far, no evidence suggests that trans women develop more aggressive prolactin tumors than cisgender women – if anything, the overall incidence is lower than might be expected given the degree of prolactin elevation.
Monitoring: Even though the risk is low, guidelines recommend periodic prolactin monitoring in trans women on estrogen, especially if also on cyproterone or if symptoms occur ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ). A common practice is to check prolactin at baseline, then annually for a few years. Mildly elevated prolactin without symptoms (e.g. <100 ng/mL) is usually observed. If levels rise substantially or symptoms of a tumor appear, an MRI of the pituitary is warranted. In practice, significant hyperprolactinemia is more frequently an issue with cyproterone. One study showed prolactin elevations returned to baseline after stopping cyproterone (post-orchiectomy), reinforcing the link ( Toward a Lowest Effective Dose of Cyproterone Acetate in Trans Women: Results From the ENIGI Study - PMC ). Trans women on spironolactone or GnRH analogues plus estradiol rarely get very high prolactin levels.
Bottom Line: Prolactin elevation is a known side effect of feminizing HRT. However, clinically relevant prolactinomas are exceedingly rare (). The risk is low, but prudent monitoring is advised. Patients should report any unexplained headaches, vision changes, or nipple discharge. Overall, fears of prolactinoma should not deter appropriate HRT use, given the low incidence – just something to be mindful of during long-term care.
Meningioma Risk with Cyproterone Acetate
Cyproterone acetate (CPA) is a powerful antiandrogen often used in transfeminine regimens (particularly in Europe). It is essentially a synthetic progestogen with anti-testosterone effects. Long-term use of high-dose cyproterone has been linked to meningiomas, which are usually benign tumors of the brain lining (meninges). This association has come to light in recent years through large epidemiological studies.
Risk Magnitude: A French nationwide cohort study in 2021 (covering over 250,000 cyproterone users) demonstrated a clear dose-dependent risk of intracranial meningioma (Cyproterone acetate and the risk of meningioma). Women who accumulated ≥3 grams of CPA (equivalent to >25 mg/day for 6+ months) had a significantly higher incidence of meningiomas than those on lower doses (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed) (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). The incidence among high-dose users was about 23.8 per 100,000 person-years vs 4.5 per 100,000 in low-dose users (a more than fivefold difference) (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). After adjusting for confounders, the hazard ratio was ~6.6-fold for the exposed group vs controls (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). Moreover, extremely high cumulative exposure (>60 g total) drove the risk even further – with an HR of ~21.7 in that subset (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). In practical terms, using 50–100 mg of cyproterone daily for several years markedly raises one’s risk of developing a meningioma.
Even transfeminine-specific data reflects this risk. In the French study, a subgroup of about 10,000 trans women on CPA showed a similarly elevated meningioma incidence (~20.7 per 100,000 person-years) (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). Meningiomas were especially noted in certain locations (skull base regions rich in hormone receptors) in CPA users (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed).
Mechanism: The biological mechanism is thought to be related to progesterone receptor activation in meningeal tissues. Meningiomas often express progesterone receptors, and cyproterone, being a progestin, can strongly stimulate these receptors (Cyproterone acetate and the risk of meningioma) (Cyproterone acetate and the risk of meningioma). This hormonal stimulation likely promotes growth of latent meningioma cells. In essence, CPA “feeds” the meningioma much like progesterone can. This is analogous to rare cases of meningioma growth seen with other high-dose progestins (some contraceptives and hormone therapies). The risk is dose and duration dependent – which is why it’s mainly seen at CPA doses ≥25 mg/day and long treatment spans (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)) (Cyproterone acetate and the risk of meningioma). Low-dose CPA (1–2 mg in birth control pills) has not shown this risk (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)).
Significance: While meningiomas are usually benign and slow-growing, they can cause serious issues (seizures, neurological deficits) and often require surgery or radiation if symptomatic. Thus, this risk is clinically significant. The observed frequency in high-dose CPA users (on the order of 1 in 4,000 users per year at highest doses (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed)) is considered rare but noteworthy. European regulators now warn that “the occurrence of (multiple) meningiomas is associated with longer-term use of cyproterone at doses of 25 mg/day or higher” (High-dose cyproterone acetate: potential risk of (multiple ... - GOV.UK) (Cyproterone acetate and the risk of meningioma). France and other countries have even restricted CPA’s indications and recommend annual neurological monitoring for those on high doses.
What Transfeminine Patients Should Know: Trans women taking cyproterone should be informed of this risk. Signs of meningioma can include headaches, changes in vision or hearing, or memory problems (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)) (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)). If such symptoms arise, imaging (MRI) is indicated. Guidelines now suggest using the lowest effective CPA dose or considering alternative blockers (see Risk Mitigation). If a trans woman on CPA is diagnosed with a meningioma, CPA must be permanently discontinued (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)) (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)), as tumors often stabilize or even regress off the drug.
In summary, prolonged high-dose CPA use confers a clear meningioma risk. Mechanistically it’s tied to progestogenic stimulation of meningeal cells. The risk is “very low” in absolute terms (estimated between 1–10 cases per 10,000 people (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)) (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA))), but given the seriousness, it warrants caution. Many clinics have shifted to other antiandrogens or reduced CPA dosing as a result.
Bone Health (Bone Density and Osteoporosis)
Sex hormones are crucial for bone density maintenance. In transfeminine individuals, the removal or suppression of testosterone combined with estrogen therapy creates a new hormonal milieu for bones. The net effect on bone mineral density (BMD) can vary depending on treatment details, but with proper management estrogen can protect bone health similarly to how it does in cisgender women.
Baseline Factors: Some studies suggest trans women may enter HRT with suboptimal bone metrics. One study found transfeminine patients had lower physical activity, muscle strength, and vitamin D levels than cis men (Bone health and osteoporosis | Gender Affirming Health Program) – all factors that could predispose to lower BMD even before treatment. Another analysis noted trans women had somewhat lower bone mass and cortical bone size than cis men prior to HRT, possibly due to lifelong differences in weight-bearing exercise or nutrition (Managing skeletal issues in transgender and gender ... - Mayo Clinic). These pre-existing factors mean bone health is an important consideration from the start.
Effect of HRT on BMD: Research has shown mixed outcomes:
- Some studies show BMD increases or stabilizes after estrogen therapy, presumably because estrogen prevents bone loss that would occur from hypogonadism. In trans women who start HRT without any delay after blocking testosterone, estrogen can maintain or even improve BMD, especially in the lumbar spine.
- Other studies show BMD decreases, particularly if there is a period of androgen blockade without adequate estrogen. In the past, some patients took a testosterone blocker for months before adding estradiol, leading to a hypo-estrogen, hypo-androgen state that caused bone loss. When estrogen is introduced, BMD may recover partially but could net a slight loss.
- Many studies show no significant change in bone density over the first few years of HRT (Bone health and osteoporosis | Gender Affirming Health Program).
A systematic review encompassing various regimens found conflicting results, likely because of those protocol differences (timing of estradiol start, dosing, length of follow-up) (Bone health and osteoporosis | Gender Affirming Health Program). On balance, estrogen appears to preserve bone density in transfeminine people, akin to its role in postmenopausal HRT, provided levels are in the female physiologic range.
Critical Risk Factor – Hormone Gaps: The greatest risk to bone health is under-supply of estrogen. Known risk factors for osteoporosis in trans women include prolonged periods without hormones after gonad removal, or use of puberty blockers/androgen blockers without concomitant estrogen (Bone health and osteoporosis | Gender Affirming Health Program). If a trans woman undergoes orchiectomy (removal of testes) and either stops or significantly reduces estrogen (for instance, due to cost or other issues), she essentially enters an acute menopause and can lose bone rapidly. Similarly, a trans woman on a GnRH analog or high-dose blocker who delays starting estradiol will have low sex hormones and likely bone loss. WPATH advises avoiding any long gaps in hormone therapy for this reason.
Studies confirm that GnRH analogues alone (without estradiol) cause a drop in BMD over time (Bone health and osteoporosis | Gender Affirming Health Program). However, when estrogen is added or blockers are stopped, bone density tends to return to normal (Bone health and osteoporosis | Gender Affirming Health Program). In trans women who adhere to therapy, BMD generally remains comparable to cisgender women of the same age. In fact, small studies show trans women on stable estrogen have BMD in the osteopenic range no more frequently than cis women. Trans women also tend to have had the higher peak bone mass from male puberty, which may offer some advantage.
Comparison to Cisgender People: Compared to cis men (who have higher bone density on average), trans women’s BMD will usually decrease somewhat with transition, trending toward female norms. Compared to cis women, trans women’s bone density is similar or slightly higher (owing to larger bone size from male development). Importantly, estrogen is the key hormone for bone in all sexes – cis men rely on aromatization of testosterone to estrogen for bone maintenance. Thus, once a trans woman is on estrogen, her bone metabolism is supported much like a cis woman’s. If estrogen levels are in the normal female range, significant bone loss is not expected. Indeed, one long-term Dutch study found osteoporosis rates in older trans women were not higher than in cis women, provided they were consistently on estrogen.
Guidelines: There are no unique bone screening rules for trans people yet, but experts recommend standard osteoporosis screening (DEXA scans) by age 65 for all, and earlier (50–64) if risk factors exist (Bone health and osteoporosis | Gender Affirming Health Program). A trans woman who had an orchidectomy and then went several years without HRT should be considered high-risk and get a bone density test regardless of age (Bone health and osteoporosis | Gender Affirming Health Program). Lifestyle measures (calcium, vitamin D, exercise) are advised similar to the general population. Overall, with continuous estrogen therapy, bone mass is preserved in transfeminine individuals – interrupted or inadequate hormone therapy is the main threat to bone health (Bone health and osteoporosis | Gender Affirming Health Program).
Other Health Risks and Considerations
Beyond the major categories above, a few additional risks and observations from recent research are worth noting:
- Elevated Triglycerides and Pancreatitis: As mentioned, oral estrogen can raise triglyceride levels. In extreme cases, this has led to acute pancreatitis. There are case reports of trans women developing pancreatitis due to severe hypertriglyceridemia on high-dose estradiol (Acute pancreatitis due to hypertriglyceridaemia in a transgender ...). This is quite rare, and usually occurs in those with underlying lipid disorders. Routine lipid monitoring can catch extreme TG elevations; if noted, switching to transdermal estrogen or adjusting therapy can mitigate this risk.
- Gallbladder Disease: Estrogens increase cholesterol excretion in bile, which can promote gallstone formation. Cisgender women on estrogen (e.g. birth control or HRT) have higher gallstone risk. It’s suspected that trans women on estrogen may similarly have a modestly higher risk of gallstones and gallbladder disease. While direct studies are lacking, clinicians consider this possibility, especially if a patient has risk factors (obesity, rapid weight loss, family history). Ensuring a healthy diet and weight can help lower gallstone risk while on HRT.
- Liver Enzymes: Oral estrogens can cause mild elevations in liver enzymes. These are usually asymptomatic and not associated with liver damage. The effect is less with transdermal routes. Routine liver function tests are sometimes done; significant liver toxicity from estradiol is uncommon at the doses used for HRT (unlike old high-dose ethinyl estradiol which had more hepatic impact). Nonetheless, providers will evaluate any marked ALT/AST increases for other causes.
- Mood and Migraines: High estrogen levels or fluctuations (especially with injected estrogen) can trigger migraines in susceptible individuals. Trans women with a history of migraine may experience changes in their headache patterns on HRT. Similarly, mood swings or irritability can occur if estrogen doses are too high or if progestins are added (some report mood dips on medroxyprogesterone, for instance). Close dose titration usually manages these issues. Most psychological effects of HRT are positive (reduced gender dysphoria, anxiety, etc.), but each individual’s neuroendocrine response can vary.
- Fertility: While not a “health risk” per se, it’s important to note that estrogen + antiandrogen therapy drastically reduces fertility in trans women. Sperm production falls (often to zero over time). This is often permanent after prolonged use, though some partial recovery can occur if HRT is stopped early. Transfeminine individuals are counseled about sperm banking before starting therapy if they desire biological children in the future.
- Cancer of Male Organs: Interestingly, some risks go down. For example, prostate cancer risk is reduced in trans women due to androgen deprivation. Large cohorts show trans women have lower prostate cancer incidence than age-matched cis men (Prostate cancer in transgender women: what does a urologist need ...). Estrogen and low testosterone likely provide protection. That said, trans women can still get prostate cancer (especially if starting HRT later in life), so regular screening (e.g. PSA testing by age 50–55) is still considered for those with a prostate (Prostate cancer in transgender women - Harvard Health). Testicular cancer risk also essentially drops after orchiectomy or prolonged gonadal suppression.
- Mortality: Overall mortality among trans women on HRT has not been shown to increase from HRT itself. Some older studies raised concern about higher overall mortality, but those were confounded by factors like HIV, substance use, etc. When looking at hormone-related mortality, most data are reassuring – with the main HRT-related deaths being rare cases of thromboembolism or stroke. A Dutch study found trans women’s all-cause mortality was not higher than the general population once HIV-related deaths were excluded ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ), and that mortality could not be directly attributed to hormone treatment ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ).
It’s clear that current research continues to refine our understanding of transfeminine health risks. Most risks are manageable with proper medical supervision. Importantly, the quality-of-life and mental health benefits of gender-affirming hormone therapy are significant and must be weighed against these medical risks ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ). Informed consent and personalized care enable most trans women to navigate these risks safely.
Risk Mitigation Strategies
Despite the above risks, there are effective ways to minimize health dangers while allowing transfeminine individuals to reap the benefits of HRT. Key strategies involve optimizing the hormone regimen and addressing modifiable risk factors:
Adjust Hormone Dosages to the Lowest Effective Level
- Use the minimum effective dose of estrogen and adjuncts: Supraphysiologic doses do not speed up feminization significantly but do increase risks. Providers aim for estrogen levels in the mid-normal female range (typically ~100–200 pg/mL estradiol). Once breast development and other changes plateau (often after 2–3 years), dosing can often be tapered to a maintenance level. Using the lowest dose that achieves desired results helps reduce VTE, cardiovascular, and prolactin-related risks (since those risks tend to rise with higher hormone concentrations).
- Titrate cyproterone down or discontinue when possible: Given its meningioma and prolactin concerns, many protocols now use 50 mg or even 25 mg of CPA instead of 100 mg, or stop it after gonadectomy (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)) (Restrictions in use of cyproterone due to meningioma risk | European Medicines Agency (EMA)). Recent studies show even lower doses of CPA can effectively suppress testosterone in most trans women ( Toward a Lowest Effective Dose of Cyproterone Acetate in Trans Women: Results From the ENIGI Study - PMC ). Titrating to the lowest effective dose of CPA reduces side effects while still achieving androgen blockade ( Toward a Lowest Effective Dose of Cyproterone Acetate in Trans Women: Results From the ENIGI Study - PMC ). In patients willing, an orchiectomy (surgical removal of testes) eliminates the need for any antiandrogen and allows a reduction in estrogen dose too.
- Avoid old estrogen formulations: Ethinyl estradiol and high-dose conjugated equine estrogens (Premarin) are outdated in trans care due to their risk profile. Modern guidelines advocate 17β-estradiol (bioidentical estrogen) at moderate doses. This estrogen has a shorter half-life and a more natural metabolic profile, lowering risk of clotting and cancer. By adhering to current standards, one avoids unnecessary risk that came with older regimens (Overview of feminizing hormone therapy | Gender Affirming Health Program).
- Monitor hormone levels and adjust: Regular blood tests to ensure estradiol isn’t excessively high (especially with injectable or high-dose oral forms) can prevent inadvertent overdose. If levels are supra-physiological, dose should be reduced. Likewise, periodic checks that testosterone is sufficiently suppressed can inform if antiandrogen dosing can be lowered (e.g. if T is already low, one might not need as much blocker).
Prefer Safer Routes and Medication Options
- Transdermal estrogen is preferred for those at VTE risk: Applying estrogen through the skin (patches, gels) avoids liver first-pass and has minimal impact on clotting factors (Overview of feminizing hormone therapy | Gender Affirming Health Program). Data from menopausal women show no increased VTE risk with transdermal estradiol (Overview of feminizing hormone therapy | Gender Affirming Health Program), and trans women on transdermal formulations have very low incidence of clots. Thus, transdermal estrogen is recommended for patients over ~40, smokers, or anyone with elevated thrombosis risk (Overview of feminizing hormone therapy | Gender Affirming Health Program). Many clinicians use patches as first-line for older transfeminine patients. If oral estrogen is used, switching to transdermal should be considered if any clot or cardiovascular issues develop.
- Consider injectable estradiol valerate/cypionate: Intramuscular or subcutaneous injections of estradiol avoid first-pass metabolism as well. They do produce higher peaks, but overall have a lower impact on clotting than oral dosing. Some patients prefer injections (biweekly or weekly). Care is taken to use reasonable doses to avoid extreme hormone peaks.
- Choose anti-androgens wisely: Spironolactone (a potassium-sparing diuretic with antiandrogen effects) is commonly used in the US. It does not carry the meningioma or prolactin risk that cyproterone does. Though Spiro can cause high potassium or hypotension, those are manageable with monitoring. GnRH analogs (like leuprolide) effectively shut down testosterone and are very safe long-term (aside from cost), essentially putting the patient in a reversible “castrate” hormonal state. These are great options, especially for those who cannot tolerate other blockers. Lower-risk progestins: If a progestogen is desired for potential breast development benefits, micronized progesterone may be used. Micronized (bioidentical) progesterone is thought to have a more benign risk profile on blood clots and mood than synthetic progestins (like medroxyprogesterone) (Overview of feminizing hormone therapy | Gender Affirming Health Program) (Overview of feminizing hormone therapy | Gender Affirming Health Program). Though not proven in trans populations, it’s an option some providers consider for those requesting a progestogen.
- Regularly review the regimen: Over the course of transition, medical needs change. For instance, after genital surgery or years on HRT, testosterone production is minimal – at that point, continuing a high dose antiandrogen is unnecessary and only adds risk. Thus, clinicians periodically deprescribe medications that are no longer needed or adjust doses downward. This ongoing optimization keeps the hormone therapy “lean” and safe.
Lifestyle and Preventive Health Interventions
- Smoking cessation: Smoking dramatically increases cardiovascular and thrombotic complications in estrogen users. Every effort should be made to help transfeminine patients quit smoking (Overview of feminizing hormone therapy | Gender Affirming Health Program). This includes counseling, nicotine replacement, or medications as needed. If a patient continues to smoke, one can still provide HRT (as outright denial could cause other harms), but in such cases using transdermal estrogen and perhaps even adding prophylactic aspirin (low-dose) is considered (Overview of feminizing hormone therapy | Gender Affirming Health Program). (Note: low-dose aspirin for prevention is debated and should be individualized (Overview of feminizing hormone therapy | Gender Affirming Health Program)).
- Weight management and diet: A healthy diet and regular exercise are important to counteract weight gain and insulin resistance from HRT. Trans women should be encouraged to keep an eye on calorie intake, focusing on balanced nutrition with adequate protein (to mitigate muscle loss) and plenty of fruits/vegetables. Exercise – particularly weight-bearing and resistance exercise – can help maintain muscle mass and bone density, reducing osteoporosis risk and improving metabolic health. Even light aerobic exercise improves circulation and can lower VTE risk.
- Routine health screenings: Transfeminine individuals should receive routine screenings appropriate to their age and anatomy. This includes breast cancer screening (e.g. mammograms as indicated) since long-term estrogen exposure does confer some breast cancer risk (albeit lower than cis women) (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed). Prostate screening is still relevant (typically starting at age 50, or earlier if family history), because the prostate remains unless surgically removed. Monitoring blood pressure, glucose, and lipids regularly allows early intervention if problems arise (e.g. starting antihypertensives or metformin for pre-diabetes). Keeping these risk factors controlled will substantially lower chances of heart attacks or strokes on HRT.
- Calcium/Vitamin D and bone health: Ensuring sufficient calcium and vitamin D intake (through diet or supplements) is recommended for all, but especially if there are any concerns about bone density. Combined with exercise, this supports skeletal strength. If a patient cannot take estrogen for a time (say, post-surgery or due to another illness), supplementing with calcium/vitamin D and possibly a short-term bisphosphonate could be considered to protect bone mass until estrogen is resumed.
- Regular follow-up and labs: Continuous healthcare follow-up is itself a protective strategy. Trans women on HRT benefit from check-ups every 6–12 months, especially in the first few years. At these visits, side effects can be reviewed and labs drawn (as appropriate: lipid panel, metabolic panel, prolactin, etc.). This proactive monitoring can catch any emerging issues (like rising prolactin or liver enzymes) early, allowing for adjustments before a mild issue becomes a serious complication ( Effects of antiandrogens on prolactin levels among transgender women on estrogen therapy: A systematic review - PMC ).
- Patient education: Informing patients about symptoms of possible complications empowers them to seek help early. They should know to watch for leg swelling/pain (possible DVT), chest pain or difficulty breathing (possible PE or cardiac issue), neurological symptoms (possible stroke or rarely meningioma), etc. Educating that “if you experience X, call us immediately” can be lifesaving. Patients should also understand the importance of adherence – irregular dosing can cause hormonal swings that might exacerbate risks (for example, missed doses of estrogen after orchiectomy could lead to bone loss).
In conclusion, risk mitigation is about smart prescribing and holistic care. By using safer hormone regimens (correct dose, route, and medications) (Overview of feminizing hormone therapy | Gender Affirming Health Program) and addressing lifestyle factors, providers can significantly lower the risks of feminizing hormone therapy. Modern medical guidelines emphasize that with proper oversight, hormone therapy for transfeminine individuals can be administered safely and effectively, with risks comparable to those of cisgender hormone therapies (Overview of feminizing hormone therapy | Gender Affirming Health Program). The goal is to maximize gender-affirming benefits while minimizing adverse outcomes – something very much achievable with today’s knowledge and a proactive approach.
Sources:
- de Blok CJM et al. (2019). Breast cancer risk in transgender people receiving hormone treatment: Nationwide cohort study. BMJ, 365, l1652. – Incidence of breast cancer in trans women vs cis men/women (Breast cancer risk in transgender people receiving hormone treatment: nationwide cohort study in the Netherlands - PubMed).
- Wierckx K et al. (2013). Venous thrombosis and changes in coagulation profile during cross-sex hormone therapy in trans people. J Sex Med, 10(12), 3129-37. – Highlights estrogen-induced VTE risk and importance of route (transdermal vs oral) ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ) (Overview of feminizing hormone therapy | Gender Affirming Health Program).
- Getahun D et al. (2018). Cross-sex Hormones and Acute Cardiovascular Events in Transgender Persons. Ann Intern Med, 169(4), 205-213. – Cohort study on VTE, stroke, MI in trans populations (trans women had higher VTE; stroke/MI risk differed vs cis men/women) (Cross-sex Hormones and Acute Cardiovascular Events in Transgender Persons: A Cohort Study - PubMed) (Balancing the Cardiovascular Risks of Hormone Therapy for Transgender Patients | GE HealthCare (United States)).
- Baker KE et al. (2021). Hypercoagulability and Cardiovascular Risk during Gender-Affirming Hormone Therapy. Clin Chem, 67(1), 132-142. – Review of thrombosis mechanisms in GAHT; notes oral estrogen ~3-fold VTE risk vs cis women, transdermal no increase ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ).
- Hembree WC et al. (2017). Endocrine Treatment of Gender-Dysphoric/Gender-Incongruent Persons: Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab, 102(11), 3869-3903. – Standard guidelines; advise prolactin monitoring and note prolactinoma risk is very low (only rare cases reported) ().
- Nota NM et al. (2019). Prolactin in transgender women after orchiectomy. Andrologia, 51(10), e13404. – Found prolactin levels drop to baseline after stopping cyproterone post-orchiectomy ( Toward a Lowest Effective Dose of Cyproterone Acetate in Trans Women: Results From the ENIGI Study - PMC ). Confirms CPA’s role in elevating prolactin.
- Donato D et al. (2020). Cyproterone acetate and risk of intracranial meningioma. BMJ, 371, m4457. – French cohort: dose-dependent meningioma risk with CPA (HR ~6 for >3g; ~22 for >60g cumulative) (Use of high dose cyproterone acetate and risk of intracranial meningioma in women: cohort study - PubMed). Led to EU warnings (Cyproterone acetate and the risk of meningioma).
- UCSF Transgender Care Guidelines (2016). Primary Care Protocol for Transgender Patient Care. – Recommends transdermal estrogen for those >40 or with VTE risk, and tobacco cessation counseling for all trans women on HRT (Overview of feminizing hormone therapy | Gender Affirming Health Program) (Overview of feminizing hormone therapy | Gender Affirming Health Program). Also discusses bone health screening and maintaining post-gonadectomy hormones (Bone health and osteoporosis | Gender Affirming Health Program).
- World Prof. Assoc. for Transgender Health (WPATH) Standards of Care, 8th Version (2022). – Emphasizes need for ongoing monitoring (lipids, glucose, prolactin) and risk reduction in hormone therapy. Notes benefits outweigh risks when managed properly ( Gender-affirming hormone therapy in the transgender patient: influence on thrombotic risk - PMC ).
- Cheung AS et al. (2020). Effects of GAHT on insulin resistance and body composition in transgender individuals: Systematic review. World J Diabetes, 11(3), 66-77. – Summarizes that trans women on HRT lose lean mass, gain fat, and may worsen insulin resistance ( Effects of gender-affirming hormone therapy on insulin resistance and body composition in transgender individuals: A systematic review - PMC ), underlining need to watch metabolic health.